Hesperidin has the functions of maintaining osmotic pressure, enhancing capillary toughness, shortening bleeding time, and lowering cholesterol. It is clinically used in the adjuvant treatment of cardiovascular diseases. It can cultivate a variety of drugs to prevent arteriosclerosis and myocardial infarction.
It can be used as a natural antioxidant in the food industry. It can also be used in the cosmetics industry. Hesperidin is mostly found in the waste of citrus processing, such as the peel and sac, and the content of hesperidin in the mature peel and tissue* (endocarp 30%-50%, orange network, stone, pulp 30%-50% in the middle, 10%-20% in the exocarp), and 1%-5% in the juice and orange
The extraction methods of hesperidin include solvent extraction, alkaline extraction and acid precipitation, carbon powder adsorption, and ion exchange. Among them, the alkaline extraction and acid precipitation method has simple operation, low cost and high extraction rate. Orange peel is soluble in dilute alkali and pyridine and hot water above 70℃, slightly soluble in methanol, and almost insoluble in acetone, benzene and chloroform.
The extraction of hesperidin mainly uses the two phenolic hydroxyl groups contained in it to react with sodium ions in the solution under alkaline conditions to form sodium salt and dissolve it, then acidify and cool to make it precipitate out of the solution. The extraction of hesperidin from citrus peel generally adopts hot extraction method and soaking extraction method, and the outcome is not ideal. In recent years, ultrasonic extraction of active ingredients from natural plants (especially Chinese herbal medicines) has been extensively carried out, and certain progress has been made.
Attention
Use hesperidin for extraction under alkaline conditions by ring-opening dissolution, and separation under acidic conditions by closed-loop precipitation. Increasing the amount of alkali during extraction can reduce the amount of ethanol, but the alkalinity should not be too large, otherwise, hesperidin will be easily oxidized damage.
